Test antifungal activity Atsiri Flower Oil Cengkeh (Syzygium aromaticum (L.) Merr et Perry) on Candida albicans in vitro and GC-MS Profile

Indonesia has many kinds of plants which used as a traditional medicine, one of them is flower of clove ( Syzygium aromaticum (L) Merr et Perry ) that is used as a local anaesthetic, antiseptic and aromatic substance. The one of use of that flower of clove is probably relationship with the antifungal activity. So, it is necessary to do an experiment about activity test of antifungal from flower of clove.

It had been conducted a research about flower of clove ( Syzygium aromaticum (L) Merr et Perry ) essense oil as antifungal againts Candida albicans and analysis the component using GC-MS. The objectives of research was to determine the activities of the essential oil of flower of clove ( Syzygium aromaticum (L) Merr et Perry ) to the growth of Candida albicans by identifying the Minimum Inhibiting Concentration (MIC) and the Minimum Fungicide Concentration (MFC) and to analyze the essential oil content of flower of clove.

The essential oil of flower of clove was isolated using water and steam destilation method. The qualitative analysis of the essential oil conducted organoleptically, including its smell, taste and color, as well as refractive index. The essential oil composition was analyzed using GC-MS Shimadzu-QP2010S. The antifungal activity test was done using the liquid dilution method. To determine MIC againts C. albicans had made various concentration of the flower of clove ( Syzygium aromaticum (L) Merr et Perry ) esense oil 0,1 %v/v ; 0,05 %v/v ; 0,025 %v/v ; 0,0125 %v/v ; 0,00625 %v/v and 0,003125 %v/v.

The Minimum Fungicide Concentration (MFC) was determined by scratching the tested solution of the Minimum Inhibiting Concentration (MIC) examination on the Sabouraud Dextrose Agar (SDA). The MIC was indicated by the lowest concentration that still inhibit the fungal growth, where as the minimum fungicidal was indicated by the lowest concentration that still able to kill fungi. The result of this research was indicated that the flower of clove ( Syzygium aromaticum (L) Merr et Perry ) essense oil have antifungal activity againts C. albicans.The MIC can’t identified, but MFC of ( Syzygium aromaticum (L) Merr et Perry ) esense oil value was 0,025 %v/v. The estimation result of chemical component analysis by GC-MS was indicated that ( Syzygium aromaticum (L) Merr et Perry ) esense oil contained of eugenol, trans – caryophyllene, alpha – humulene and eugenol acetate.

research by
Dwi Hani Lestari